The modern state of Pakistan was established on 14 August 1947, but the region it encompasses has an extensive history that overlaps with the histories of Ancient India, Iran and Afghanistan. The region was a crossroads of historic trade routes, including the Silk Road, and was settled over thousands of years by many groups, including Dravidians, Indo-Aryans, Persians, Greeks, Scythians, Parthians Kushans, White Huns, Afghans, Arabs, Turks, and Mongols; the region is often referred to as "a museum of races."

 Historian and geographer de Blij Muller characterized the historical embodiment of the land when he said, "If, as is so often said, Egypt is the gift of the Nile, then Pakistan is the gift of the Indus." The earliest evidence of humans are pebble tools from the Soan Culture in the province of Punjab, dated from 100,000 to 500,000 years ago. The Indus region was the site of several ancient cultures including Mehrgarh, one of the world's earliest known towns, and the Indus Valley Civilisation at Harrappa and Mohenjo-Daro.

The Indus Valley Civilisation collapsed in the middle of the second millennium BCE and was followed by the Vedic Civilisation, which extended over much of northern India and Pakistan. Successive empires and kingdoms ruled the region from the Achaemenid Persian empire around 543 BCE, to Alexander the Great in 326 BCE and the Mauryan empire. The Indo-Greek Kingdom founded by Demetrius of Bactria included Gandhara and Punjab from 184 BCE, and reached its greatest extent under Menander, establishing the Greco-Buddhist period with advances in trade and culture. The city of Taxila (Takshashila) became a major centre of learning in ancient times - the remains of the city, located to the west of Islamabad, are one of the country's major archaeological sites.